Potexvirus gene expression

images potexvirus gene expression

Viral vector systems were predominantly developed for transient expression followed by infection Donson at al. Sit, T. A variant of said potexviral coat protein is considered a function-conservative variant of said coat protein if it is capable of providing said RNA replicon, together with other necessary components such as the TGB, with the capability of cell-to-cell movement in a plant or in plant tissue. Said nucleic acid sequence of item ii may comprise a further sequence encoding a protein necessary for cell-to-cell movement of said RNA replicon such as a potexviral coat protein or a function-conservative variant thereof. For being an RNA replicon, i. BRPIA2 en. If two or more proteins of interest are to be produced in the same plant or in the same plant tissue, said plant or plant cells may be provided with a second nucleic acid comprising or encoding a second or further RNA replicon. Chapman, S. In another embodiment of this invention, the effect of the position of PVX CP preceding the gene of interest was tested. In the special case of potexviral vectors, this vector design leads to a cell-to-cell movement capability of the RNA replicon and, at the same time, to higher expression levels of the heterologous nucleic acid compared to conventional potexviral vectors where a heterologous nucleic acid was placed upstream of the potexviral coat protein gene.

  • ORF3 Movement protein TGB2 Papaya mosaic potexvirus (PMV) ORF3 gene & protein
  • USB2 Potexvirusderived replicon Google Patents
  • USB2 Potexvirusderived replicon Google Patents

  • GENE EXPRESSION. The virion RNA is infectious and serves as both the genome and viral messenger RNA. RNA-dependent RNA polymerase (RdRp) is.

    Efficiency of VIGS and gene expression in a novel bipartite potexvirus vector delivery system as a function of strength of TGB1 silencing. Viral and Nonviral Elements in Potexvirus Replication and Movement and in . Gene expression is thought to be similar to that in other potexviruses, that is, the.
    Processes and vectors for amplification or expression of nucleic acid sequences of interest in plants. If said nucleic acid is DNA, it will typically have a transcription promoter for allowing production by transcription of said RNA replicon in vitro or in planta.

    The invention further provides a kit of parts comprising at least two vectors pro-vectors that are capable of assembling said nucleic acid of the invention in plant cells by site-directed recombination. Fedorkin, O.

    images potexvirus gene expression

    In another embodiment, Nicotiana species like Nicotiana benthamiana and Nicotiana tabacum are used; preferred plant species other than Nicotiana species are Petunia hybrida, Brassica campestris, B.

    Therefore, the coat protein gene of potexvirus-derived viral vectors cannot be removed without a severe loss of protein expression efficiency.

    images potexvirus gene expression
    Potexvirus gene expression
    The process of the invention is preferably performed in plants or in plant tissue.

    BRPIA2 en. However, two or more different RNA replicons may be used in a plant or plant tissue for expressing two different proteins of interest, whereby such different RNA replicons are preferably derived from different plant viruses.

    images potexvirus gene expression

    Potexviruses are plant RNA viruses with a plus-sense single-stranded genome. Becker et al.

    Alternanthera mosaic potexvirus: Several Features, Properties, and. for target protein expression in plants and virus-induced gene silencing. DeltaCP lacking the entire CP gene, was studied by transient co-expression with heterologous proteins [23]. gene block proteins, as revealed by a trans- complementation assay based on the bamboo mosaic virus satellite RNA-mediated expression system.

    Ming-Kuem .
    Examples of such proteins of interest include inter alia the following proteins pharmaceutical proteins : immune response proteins monoclonal antibodies, single chain antibodies, T cell receptors etc. Two or more vectors may be provided to a plant or to plant tissue by providing mixtures of the vectors or mixtures of Agrobacterium strains, each strain containing one of said vectors, to a plant or to plant tissue.

    In the special case of potexviral vectors, this vector design leads to a cell-to-cell movement capability of the RNA replicon and, at the same time, to higher expression levels of the heterologous nucleic acid compared to conventional potexviral vectors where a heterologous nucleic acid was placed upstream of the potexviral coat protein gene. Said further RNA replicon may then encode one or more further proteins of interest.

    Therefore, the coat protein gene of potexvirus-derived viral vectors cannot be removed without a severe loss of protein expression efficiency.

    ORF3 Movement protein TGB2 Papaya mosaic potexvirus (PMV) ORF3 gene & protein

    This object is further solved by a nucleic acid comprising or encoding an RNA replicon comprising, in this order, the following segments:. AUA en.

    Video: Potexvirus gene expression Inhibition of Poxvirus Gene Expression and Genome Replication by Bisbenzimide Derivatives

    images potexvirus gene expression
    Potexvirus gene expression
    Marconi, et al. Alternatively, these sequence identities may be present within a protein sequence segment of at least amino acid residues, within a protein sequence segment of at least amino acid residues, within a protein sequence segment of at least amino acid residues, or within a protein sequence segment of at least amino acid residues.

    AUB2 en. The PVX coat protein is necessary not only for systemic, but also together with three other proteins of triple gene block for cell-to-cell short distance movement Yang et al. The choice of the transformation method depends inter alia on the plant species to be transformed.

    Plant Path. The process of the invention has better biosafety features than conventional potexvirus-derived RNA replicons, as it produces less viral coat protein and consequently, can form less viral particles.

    Several new deconstructed vectors based on a potexvirus genome sequence for efficient expression of heterologous proteins in plants were. Organism.

    Papaya mosaic potexvirus (PMV).

    USB2 Potexvirusderived replicon Google Patents

    Status Triple gene block 2 protein. Short name: TGBp2 . Keywords - Technical termi. Complete proteome. The present invention relates to a process of high-yield expression of a gene of interest in a plant, in plant tissue or in plant cells using a potexvirus-derived.
    If said nucleic acid of the invention is RNA, it may be used for infecting a plant or plant tissue, preferably in combination with mechanical injury of infected plant tissue such as leaves.

    The invention also provides a process of expressing a heterologous nucleic acid sequence of interest in a plant or in plant tissue, comprising providing a plant or plant tissue with two or more vectors that are capable of assembling said nucleic acid of the invention in plant cells by site-directed recombination.

    The PVX coat protein is necessary not only for systemic, but also together with three other proteins of triple gene block for cell-to-cell short distance movement Yang et al.

    USB1 en. CAA1 en.

    USB2 Potexvirusderived replicon Google Patents

    Said RNA replicon may be built on a potexvirus and uses the replication and expression system of a potexvirus for expressing a heterologous nucleic acid sequence. USB1 en.

    images potexvirus gene expression
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    A variant of said potexviral coat protein is considered a function-conservative variant of said coat protein if it is capable of providing said RNA replicon, together with other necessary components such as the TGB, with the capability of cell-to-cell movement in a plant or in plant tissue.

    Processes and vectors for amplification or expression of nucleic acid sequences of interest in plants.

    Video: Potexvirus gene expression Day 5 DNA viruses gene expression strategies

    This object is further solved by a nucleic acid comprising or encoding an RNA replicon comprising, in this order, the following segments:. The invention provides the first high-yield potexviral plant expression system that can be used on a large scale. Hammond, J. In addition, expression of PVX coat protein in transgenic tobacco plants rescues movement-deficient PVX, but compromises the efficiency of cell-to-cell movement and viral replication Spillane et al.

    4 thoughts on “Potexvirus gene expression

    1. In the special case of potexviral vectors, this vector design leads to a cell-to-cell movement capability of the RNA replicon and, at the same time, to higher expression levels of the heterologous nucleic acid compared to conventional potexviral vectors where a heterologous nucleic acid was placed upstream of the potexviral coat protein gene.

    2. Nucleic acid comprising or encoding an RNA replica comprising, in this order, the following segments i to iii : i a nucleic acid sequence encoding a potexvirus RNA-dependent RNA polymerase or a function-conservative variant thereof; ii a nucleic acid sequence comprising: a a potexvirus triple gene block or a function-conservative variant thereof and b a sequence encoding a potexviral coat protein or a function-conservative variant thereof; or a sequence encoding a tobago viral movement protein; and iii a heterologous nucleic acid sequence expressible from said replica in a plant or in plant tissue.